Nickel HRP Conjugate – 1.0 mg
PRODUCT CODE: X-CON-0010-1MG
STORAGE: 2 – 8 °C, protected from sun light.
Metal affinity interaction allows polyhistidine tag containing protein to be complexed and detected with molecules that contain chelated divalent ions such as Ni2+, Cu2+, Co2+ and Zn2+.
Horseradish peroxidase (HRP) oxidizes corresponding substrates with high efficiency, generating colorimetric or chemiluminescent reactions and is frequently used as a reporter enzyme for sensitive assays like ELISA, immunohistochemistry and western blot. HRP is conjugated with chelated Ni2+ under optimal conditions. Nickel HRP Conjugate is useful as a reagent for detecting polyhistidine tag containing proteins in ELISA and western-blotting procedures.
PRECAUTIONS AND DISCLAIMER
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
For shipping at ambient temperature Nickel HRP Conjugate is dried with a HEPES, NaCl, sucrose buffer base.
PREPARTION AND HANDLING
The product should be reconstituted with
100 µl water yielding a concentration of
10 mg/ml. The reconstituted stock solution can be frozen in aliquots for later usage. Stock solutions can be diluted in buffers containing > 0.1 % BSA as needed. Avoid using buffer that contain EDTA or other metal chelators, avoid reducing agents, sodium acid and imidazole.
STORAGE / STABILITY
For long term storage the dry-stabilized Nickel HRP Conjugate should be stored between 2 °C and 8 °C. Reconstituted stock solutions can be stored at 2 – 8 °C for up to 2 weeks. For long term storage, stock solutions can be frozen in working aliquots. Repeated freeze-thaw cycles should be avoided.
RECONSTITUTION AND CONCENTRATION
10 mg/ml after reconstitution with 100 µl H2O.
RECOMMENDED ELISA DILUTION
1:500 – 1: 5000 in secondary ELISA detection. For optimal performance the reagent should be titrated for each application.
RECOMMENDED RETEST DATE
- Wong, J., et al., Direct force measurements of the streptavidin –biotin interaction, Biomolecular Engineering, 16, 45-55 (1999).
- Hochuli, E., et al., New metal chelate adsorbent for proteins and peptides containing neighbouring histidine residues, J. Chromatogr., 411, 177-84 (1987).