Protein A FITC Conjugate – 1.0 mg

85,00 excl. Vat

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Protein A FITC Conjugate – 1.0 mg
PRODUCT CODE: X-CON-0006-1.0MG
STORAGE: 2 – 8 °C, protected from sun light
PRODUCT DESCRIPTION
Protein A is a 42 kDa surface protein originally
found in the cell wall of the bacteria
Staphylococcus aureus. Protein A binds
strongly to the Fc region of IgG from human
(total IgG, IgG1, IgG2, IgG4), mouse (IgG2a,
IgG2b and IgG3), rabbit, guinea pig, pig, dog,
and rhesus monkey.
Fluorescein isothiocyanate (FITC) is the
derivative of fluorescein with an
isothiocyanate reactive group, rendering it
reactive towards amine and sulfhydryl groups
commonly found in biomolecules. In water, its
maximum absorption/emission is 494/518 nm,
corresponding to the colour blue and green.
The blue colour spectrum is 450-495 nm, and
the green spectrum is 495-570 nm. Protein A
is conjugated with FITC under optimal
conditions. The solution is free from unbound
FITC. Protein A FITC Conjugate is useful as a
secondary reagent for detecting biotinylated
molecules in immunohistochemistry
procedures.
PRECAUTIONS AND DISCLAIMER
This product is for R&D use only, not for drug,
household, or other uses. Please consult the
Material Safety Data Sheet for information
regarding hazards and safe handling practices.
FORMULATION
For shipping at ambient temperature Protein
A FITC conjugate is dried with a HEPES, NaCl,
sucrose buffer base.
PREPARTION AND HANDLING
The product should be reconstituted with
100 µl water yielding a concentration of
10 mg/ml. The reconstituted stock solution
can be frozen in aliquots for later usage. Stock
solutions can be diluted in buffers containing
> 0.1 % BSA (IgG free) as needed.
STORAGE / STABILITY
For long term storage the dry-stabilized
Protein A FITC Conjugate should be stored
between 2 °C and 8 °C. Reconstituted stock
solutions can be stored at 2 – 8 °C for up to 2
weeks. For long term storage, stock solutions
can be frozen in working aliquots. Repeated
freeze-thaw cycles should be avoided.
RECONSTITUTION AND CONCENTRATION
10 mg/ml after reconstitution with 100 µl H2O
(concentration relates to the Protein A only
component of the conjugate).
RECOMMENDED DILUTION
For immunofluorescent staining a final
concentration of 5 to 10 µg/ml is
recommended. For optimal performance the
reagent should be titrated for each
application.
RECOMMENDED RETEST DATE
07/2021
BACKGROUND REFERENCES
1. Goding, J.W., Use of staphylococcal protein
A as an immunological reagent, J. Immunol.
Methods, 20, 241-53 (1978)