Protein A – 5 mg
PRODUCT CODE: X-PRO-0002-5MG
STORAGE: 2 – 8 °C
Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. Protein A binds strongly to the Fc region of IgG from human (total IgG, IgG1, IgG2 and IgG4), mouse (IgG2a, IgG2b and IgG3), rabbit, guinea pig, pig, dog, and rhesus monkey.
For convenient amino-based conjugation, the product is free from amino-based puffers. Protein A is useful as reagent for coating or detecting specified IgG in ELISA and immunoblotting procedures.
PRECAUTIONS AND DISCLAIMER
This product is for LABORATORY RESEARCH USE ONLY, not for diagnostic, therapeutic, drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
For shipping at ambient temperature Protein A is dried with a HEPES, NaCl and sucrose buffer base.
PREPARTION AND HANDLING
The product should be reconstituted with 100 µl water yielding a concentration of 10 mg/ml. The reconstituted stock solution can be frozen in aliquots for later usage. Stock solutions can be diluted in appropriate buffers as needed.
STORAGE / STABILITY
For long term storage the dry-stabilized Protein A should be stored between 2 °C and 8 °C. Reconstituted stock solutions can be stored at 2 – 8 °C for up to 2 weeks. For long term storage, stock solutions can be frozen in working aliquots. Repeated freeze-thaw cycles should be avoided.
RECONSTITUTION AND CONCENTRATION
10 mg/ml after reconstitution with 100 µl H2O.
For protein conjugation a final concentration of 2 to 10 mg/ml is recommended. For direct microplate coating a final concentration of 0.5 to 5 µg/ml is recommended. For optimal performance the reagent should be titrated for each application.
RECOMMENDED RETEST DATE
1. Goding, J.W., Use of staphylococcal protein A as an immunological reagent, J. Immunol. Methods, 20, 241-53 (1978).
CERTIFICATE OF ANALYSIS
APEARANCE (COLOR): clear to white
APEARANCE (FORM): dried film
STORAGE 2-8 °C, protected from prolonged exposure to light
FORMULATION: Protein A dried with HEPES, NaCl, Sucrose buffer base
CONCENTRATION: 1.0 mg/ml after reconstitution with 100 µl H2O
RECOMMENDED RETEST DATE: 07/2021