SARS-CoV-2 Biotin-Spike Protein S1 – 1.0 mg
PRODUCT CODE: X-PRO-00008-1.0MG
ALIQUOTING: 1 X 1.0 mg vial
STORAGE: < -18 °C
The SARS-CoV-2 spike (S) protein is a large type I transmembrane protein composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2).
PRECAUTIONS AND DISCLAIMER
This product is for LABORATORY RESEARCH USE ONLY, not for diagnostic, therapeutic, drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
For shipping at ambient temperature the protein is liquid-stabilized with a HEPES, NaCl, small molecule buffer base at neutral pH.
PREPARATION AND HANDLING
Stock solutions can be diluted in appropriate biotin free buffers.
STORAGE / STABILITY
The E. coli expressed recombinant SARS-CoV-2 Spike Protein S1, full length S1 fragment, aas 13-685, has a molecular mass of 75 kDa and a predicted isoelectric point of 8,2. Spike-1 Protein is conjugated with biotin under optimal conditions.
The E. coli expressed recombinant SARS-CoV-2 Nucleocapsid Protein has a molecular mass of 47 kDa and a predicted isoelectric point of 10. Nucleocapsid Protein is conjugated with biotin under optimal conditions.
For direct microplate coating a final concentration of 0.5 to 5 μg/ml is recommended. For optimal performance the reagent should be titrated for each application.
1. Walls A.., et al., Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein, Cell, 181, 281-292 (2020).
2 Lan J., et al. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor, Nature. 581: 215-220. (2020).
BioThinx warrants, that at the time of the quality release or subsequent retest date this product conformed to the information contained in this publication. Purchaser must determine the suitability of the product(s) for its particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. No other warranties, express or implied, are granted, including without limitation, implied warranties of merchantability, fitness for any particular purpose, or non-infringement