SARS-CoV-2 Spike S1 Receptor Binding domain – 1.0 m
PRODUCT CODE: X-PRO-00004-1.0MG
ALIQUOTING: 1 X 1.0 mg vial
STORAGE: < -18 °C
The SARS-CoV-2 spike (S) protein is a large type I transmembrane protein composed of two subunits, S1 and S2. The S1 subunit contains a receptor-binding domain (aas 313- 545) responsible for binding to the host cell receptor angiotensin-converting enzyme 2 (ACE2).
For convenient amino-based conjugation, the product is free from amino-based puffers.
PRECAUTIONS AND DISCLAIMER
This product is for LABORATORY RESEARCH USE ONLY, not for diagnostic, therapeutic, drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
For shipping at ambient temperature the protein is liquid-stabilized with a HEPES, NaCl, small molecule buffer base at neutral pH.
PREPARATION AND HANDLING
Stock solutions can be diluted in appropriate buffers.
STORAGE / STABILITY
For long term storage the liquid-stabilized protein should be stored below – 18 °C. Repeated freeze-thaw cycles (> 5) should be avoided. For long term usage, stock solutions can be frozen in working aliquots.
The E. coli expressed recombinant SARS-CoV-2 Spike S1 Receptor Binding Domain, aas 313- 545, His Tag has a molecular mass of 28 kDa and a predicted isoelectric point of 8,9.
For protein conjugation a final concentration of 0.2 to 1 mg/ml is recommended. For direct microplate coating a final concentration of 0.5 to 5 μg/ml is recommended. For optimal performance the reagent should be titrated for each application.
1. Walls A.., et al., Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein, Cell, 181, 281-292 (2020).
2 Lan J., et al. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor, Nature. 581: 215-220. (2020).
BioThinx warrants, that at the time of the quality release or subsequent retest date this product conformed to the information contained in this publication. Purchaser must determine the suitability of the product(s) for its particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. No other warranties, express or implied, are granted, including without limitation, implied warranties of merchantability, fitness for any particular purpose, or non-infringement