Streptavidin – 5 mg
PRODUCT CODE: X-PRO-0001-5MG
STORAGE: 2 – 8 °C
Streptavidin binds to biotin with high affinity. The streptavidin-biotin bond is one of the strongest non-covalent interaction in nature, making it extraordinarily robust.
For convenient amino-based conjugation, the product is free from amino-based puffers. Streptavidin is useful as reagent for coating or detecting biotinylated molecules in ELISA and immunoblotting procedures.
PRECAUTIONS AND DISCLAIMER
This product is for LABORATORY RESEARCH USE ONLY, not for diagnostic, therapeutic, drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
For shipping at ambient temperature Streptavidin is dried with a HEPES, NaCl, and sucrose buffer base.
PREPARTION AND HANDLING
The product should be reconstituted with
100 µl water yielding a concentration of
10 mg/ml. The reconstituted stock solution can be frozen in aliquots for later usage. Stock solutions can be diluted in appropriate buffers.
STORAGE / STABILITY
For long term storage the dry-stabilized Streptavidin should be stored between 2 °C and 8 °C. Reconstituted stock solutions can be stored at 2 – 8 °C for up to 2 weeks. For long term storage, stock solutions can be frozen in working aliquots. Repeated freeze-thaw cycles should be avoided.
RECONSTITUTION AND CONCENTRATION
10 mg/ml after reconstitution with 100 µl H2O
For protein conjugation a final concentration of 2 to 10 mg/ml is recommended. For direct microplate coating a final concentration of 0.5 to 5 µg/ml is recommended. For optimal performance the reagent should be titrated for each application.
RECOMMENDED RETEST DATE
- Wong, J., et al., Direct force measurements of the streptavidin –biotin interaction, Biomolecular Engineering, 16, 45-55 (1999).
CERTIFICATE OF ANALYSIS
APEARANCE (COLOR): clear to white
APEARANCE (FORM): dried film
STORAGE 2 – 8 °C, protected from prolonged exposure to light
FORMULATION: Streptavidin dried with HEPES, NaCl, Sucrose buffer base
CONCENTRATION: 10 mg/ml after reconstitution with 100 µl H2O
RECOMMENDED RETEST DATE: 07/2021